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Anti-HIRA Antibody EPR7416
Also for HIRA (NM_003325)
|A synthetic peptide corresponding to residues in human HIRA was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IF
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100 - 1:500; ICC/IF: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for IHC-P.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for IHC-P.
|Homo sapiens histone cell cycle regulator (HIRA)|
|DGCR1; TUP1; TUPLE1|
|HIRA is a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this protein in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochromatin. HIRA plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate protein in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of HIRA may disrupt normal embryonic development (1).|
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Western blot - Anti-HIRA antibody [EPR7416]; All lanes : Anti-HIRA antibody [EPR7416] at 1/1000 dilution.Lane 1 : 293T cell lysates.Lane 2 : HeLa cell lysates.Lane 3 : K562 cell lysates.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution.Predicted band size : 112 kDa.
Other-Anti-HIRA antibody [EPR7416](ab129169); Equilibrium disassociation constant (KD)..
Immunocytochemistry/ Immunofluorescence - Anti-HIRA antibody [EPR7416]; ab129169, at 1/50 dilution, staining HIRA in HeLa cells by Immunofluorescence.