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Home Antibody All anti-HIF3A antibodies

Anti-HIF3A Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319371
  • Rabbit polyclonal HIF3 alpha antibody
100ug 325 3-7 Days
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WB(1)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 581-592 of mouse Hif3 a (Hypoxia Inducible Factor).
Clone Name IsotypeIgG
Species Reactivitymouse Concentration1.2 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:14,000 - 1:80,000, WB: 1:1,000 - 1:8,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three PRIVATE ''TYPE=PICT;ALT={alpha}''subunits (HIF1-aPRIVATE ''TYPE=PICT;ALT={alpha}'', HIF2-a,PRIVATE ''TYPE=PICT;ALT={alpha}'' or HIF3-a)PRIVATE ''TYPE=PICT;ALT={alpha}'' bound to the aryl hydrocarbon receptor nuclear translocator (ARNT) that is also known as HIF1-b. HIF-aPRIVATE ''TYPE=PICT;ALT={alpha}'' is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-a may be a marker for tumor growth and angiogenesis . Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive

Reference Data

Target NameMus musculus hypoxia inducible factor 3, alpha subunit (Hif3a), transcript variant 2
Alternative NamebHLHe17; Ipas; MOP7; NEPAS
Database LinkNP_058564
Entrez Gene 53417 Mouse
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using Affinity Purified anti-Hif3A antibody shows detection of a band ~72 kDa corresponding to mouse Hif3A (arrowhead).   Approximately 10 µg of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.  Treatment of exponentially growing 3T3 cells with 130 µM CoCl2 for 6 h at 37° C effectively mimics hypoxia.   After blocking the membrane was probed overnight at 4° C with the primary antibody diluted to 1:1,600.  The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

 

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