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Home Antibody All anti-HIF1AN antibodies

Anti-HIF1AN Antibody EPR3658

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307080
  • Rabbit monoclonal antibody against FIH1 (clone EPR3658 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402628) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(2)
IF(1)
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Also for HIF1AN (NM_017902)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human FIH1 was used as an immunogen.
Clone NameEPR3658 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsFC: 1:100, ICC: 1:100 - 250, IHC: 1:250 - 500, WB: 1:5,000 - 10,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN)
Alternative NameFIH1
Database LinkNP_060372
Entrez Gene 55662 Human
FunctionFactor inhibiting HIF-1 (FIH1) is an Fe (II)-dependent hydroxylase enzyme that serves as an oxygen sensor in the hypoxia response pathway (1). FIH1 binds to HIF-1alpha and inhibits its transactivation function. It also binds to VHL, which too functions as a transcriptional corepressor that inhibits HIF-1alpha transactivation function (2). In addition, FIH1 regulates the Notch signaling output and plays a role in how Notch signaling modulates the hypoxic response. It negatively regulates Notch activity and accelerates myogenic differentiation (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - HIF1AN antibody; All lanes : Anti-HIF1AN antibody [EPR3658] at 1/5000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : 293T cell lysate.Lane 3 : A375 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 40 kDa.Observed band size : 40 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HIF1AN antibody [EPR3658]; TA307080 at 1/250 dilution staining HIF1AN in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HIF1AN antibody [EPR3658]; TA307080 at 1/250 dilution staining HIF1AN in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - HIF1AN antibody [EPR3658]; TA307080 at 1/100 dilution staining HIF1AN in HeLa cells by Immunofluorescence.

 

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