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Anti-HIF1AN Antibody EPR3658
Also for HIF1AN (NM_017902)
|A synthetic peptide corresponding to residues in human FIH1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||FC: 1:100, ICC: 1:100 - 250, IHC: 1:250 - 500, WB: 1:5,000 - 10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN)|
|Factor inhibiting HIF-1 (FIH1) is an Fe (II)-dependent hydroxylase enzyme that serves as an oxygen sensor in the hypoxia response pathway (1). FIH1 binds to HIF-1alpha and inhibits its transactivation function. It also binds to VHL, which too functions as a transcriptional corepressor that inhibits HIF-1alpha transactivation function (2). In addition, FIH1 regulates the Notch signaling output and plays a role in how Notch signaling modulates the hypoxic response. It negatively regulates Notch activity and accelerates myogenic differentiation (3). |
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Western blot - HIF1AN antibody; All lanes : Anti-HIF1AN antibody [EPR3658] at 1/5000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : 293T cell lysate.Lane 3 : A375 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 40 kDa.Observed band size : 40 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HIF1AN antibody [EPR3658]; ab92498 at 1/250 dilution staining HIF1AN in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HIF1AN antibody [EPR3658]; ab92498 at 1/250 dilution staining HIF1AN in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - HIF1AN antibody [EPR3658]; ab92498 at 1/100 dilution staining HIF1AN in HeLa cells by Immunofluorescence.