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Home Antibody All anti-HIF1AN antibodies

Anti-HIF1AN Antibody EPR3659

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307079
  • Rabbit monoclonal antibody against FIH1 (clone EPR3659 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402628) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
FC(1)
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Also for HIF1AN (NM_017902)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human FIH1 was used as an immunogen.
Clone NameEPR3659 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:1000 - 1:5000; FC: 1:100 - 1:1000
Predicted MW Explanation 40 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN)
Alternative NameFIH1
Database LinkNP_060372
Entrez Gene 55662 Human
Entrez Gene 319594 Mouse
Entrez Gene 309434 Rat
FunctionFactor inhibiting HIF-1 (FIH1) is an Fe (II)-dependent hydroxylase enzyme that serves as an oxygen sensor in the hypoxia response pathway (1). FIH1 binds to HIF-1alpha and inhibits its transactivation function. It also binds to VHL, which too functions as a transcriptional corepressor that inhibits HIF-1alpha transactivation function (2). In addition, FIH1 regulates the Notch signaling output and plays a role in how Notch signaling modulates the hypoxic response. It negatively regulates Notch activity and accelerates myogenic differentiation (3).
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - HIF1AN antibody [EPR3659]; All lanes : Anti-HIF1AN antibody [EPR3659] at 1/5000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Raji cell lysate.Lane 3 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 40 kDa.
FC Image
Flow Cytometry - Anti-HIF1AN antibody [EPR3659]; Overlay histogram showing Jurkat cells stained with TA307079 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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