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Home Antibody All anti-HIF1AN antibodies

Anti-HIF1AN Antibody EPR3659

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307079
  • Rabbit monoclonal antibody against FIH1 (clone EPR3659 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402628) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
FC(1)
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Also for HIF1AN (NM_017902)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human FIH1 was used as an immunogen.
Clone NameEPR3659 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:1000 - 1:5000; FC: 1:100 - 1:1000
Predicted MW Explanation 40 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN)
Alternative NameFIH1
Database LinkNP_060372
Entrez Gene 55662 Human
Entrez Gene 319594 Mouse
Entrez Gene 309434 Rat
FunctionFactor inhibiting HIF-1 (FIH1) is an Fe (II)-dependent hydroxylase enzyme that serves as an oxygen sensor in the hypoxia response pathway (1). FIH1 binds to HIF-1alpha and inhibits its transactivation function. It also binds to VHL, which too functions as a transcriptional corepressor that inhibits HIF-1alpha transactivation function (2). In addition, FIH1 regulates the Notch signaling output and plays a role in how Notch signaling modulates the hypoxic response. It negatively regulates Notch activity and accelerates myogenic differentiation (3).
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - HIF1AN antibody [EPR3659]; All lanes : Anti-HIF1AN antibody [EPR3659] at 1/5000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Raji cell lysate.Lane 3 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 40 kDa.
FC Image
Flow Cytometry - Anti-HIF1AN antibody [EPR3659]; Overlay histogram showing Jurkat cells stained with TA307079 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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