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Home Antibody All anti-HIBCH antibodies

Anti-HIBCH TRUEMAB Antibody Clone OTI3H5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501317
  • anti-HIBCH mouse monoclonal antibody, clone OTI3H5 (formerly 3H5)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402324) , 20ug Explanation
100ul $325 In Stock
CF501317
  • Carrier-free (BSA/glycerol-free) HIBCH mouse monoclonal antibody, clone OTI3H5 (formerly 3H5)
100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IHC(3)
FC(1)
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Also for HIBCH (NM_014362)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human HIBCH (NP_055177) produced in HEK293T cell.
Clone NameClone OTI3H5 IsotypeIgG2a
Species ReactivityHuman , Dog Concentration0.9 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, FLOW 1:100
Predicted MW Explanation 39.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 1
Alternative NameHIBYLCOAH
Database LinkNP_055177
Entrez Gene 26275 Human
Entrez Gene 607040 Dog
FunctionThis gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.
Related Pathway Valine, leucine and isoleucine degradationbeta-Alanine metabolismPropanoate metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HIBCH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501317, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HIBCH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501317, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HIBCH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501317, Dilution 1:50)
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HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501317), and then analyzed by flow cytometry.
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