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Anti-HGS PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from human HRS around the phosphorylation site of tyrosine 334 (R-N-YP-W-E).|
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:1000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|HRS (Phospho-Tyr334) antibody detects endogenous levels of HRS only when phosphorylated at tyrosine 334.
|Homo sapiens hepatocyte growth factor-regulated tyrosine kinase substrate (HGS)|
TGF Beta Signaling Pathway
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Western blot analysis of extracts from HepG2 cells, treated with PMA (125ug/ml, 30mins), using HRS (Phospho-Tyr334) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using HRS (Phospho-Tyr334) antibody.The picture on the right is treated with the synthesized peptide.