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Anti-HGD Antibody EPR7874
|A synthetic peptide corresponding to residues in human HGD was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens homogentisate 1,2-dioxygenase (HGD)|
|HGD is the enzyme homogentisate 1,2 dioxygenase. It is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in HGD are the cause of the autosomal recessive metabolism disorder alkaptonuria (1).|
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Western blot - Anti-HGD antibody [EPR7874]; All lanes : Anti-HGD antibody [EPR7874] at 1/1000 dilution.Lane 1 : Caco-2 cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : Human fetal liver lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 50 kDa.Observed band size : 50 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody [EPR7874]; Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HGD with ab131035 antibody at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-HGD antibody [EPR7874]; Immunofluorescence analysis of HepG2 cells labelling HGD with ab131035 antibody at 1/100 dilution.