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Anti-HES1 Antibody EPR4226
Also for HES1 (NM_005524)
|A synthetic peptide corresponding to residues in human HES1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens hes family bHLH transcription factor 1 (HES1)|
|bHLHb39; HES-1; HHL; HRY|
|HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. HES1 has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box (1).|
Delta-Notch Signaling Pathway
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Western blot - Hes1 antibody [EPR4226]; All lanes : Anti-Hes1 antibody [EPR4226] at 1/1000 dilution.Lane 1 : Fetal brain lysate.Lane 2 : PC12 cell lysate.Lane 3 : SH-SY5Y cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 30 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hes1 antibody [EPR4226]; ab108937 at 1/250 dilution staining Hes1 in paraffin-embedded Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Flow Cytometry - Anti-Hes1 antibody [EPR4226]; Overlay histogram showing SH-SY5Y cells stained with ab108937 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.