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Anti-HDAC2 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA325112 Rabbit polyclonal HDAC2 Antibody (Center) 400ul $325 In Stock
LC419878 HDAC2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenThis HDAC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the Central region of human HDAC2.
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Mouse, Chicken) Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000, IHC: 1:50~100, FC: 1:10~50, IF: 1:10~50
Predicted MW Explanation 55364 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens histone deacetylase 2 (HDAC2), transcript variant 1
Alternative NameHD2; RPD3; YAF1
Database LinkNP_001518
Entrez Gene 3066 Human
Entrez Gene 15182 Mouse
Related PathwayStem cell - PluripotencyTranscription FactorsDruggable Genome Cell cycleNotch signaling pathwayHuntington's diseasePathways in cancerChronic myeloid leukemia

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HDAC2 Antibody (Center) (Cat. #TA325112) western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the HDAC2 antibody detected the HDAC2 protein (arrow).

IHC Image
HDAC2 Antibody (Center) (Cat. #TA325112) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HDAC2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

IF Image
IF image of Hela cell stained with HDAC2 Antibody (Center)(Cat#TA325112).Hela cells were incubated with HDAC2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . HDAC2 immunoreactivity is localized to Nucleus significantly.

FC Image
HDAC2 Antibody (Center) (Cat. #TA325112) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


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