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Anti-HDAC2 Antibody Y461
Also for HDAC2 (NM_001527)
| A synthetic peptide corresponding to residues in the C-term of human Histone Deacetylase 2 was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:2000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:60 - 1:100; IP: 1:60; IHC-Fr: 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - HDAC2 antibody [Y461]; Anti-HDAC2 antibody [Y461] at 1/2000 dilution + K562 cell lysate.Predicted band size : 55 kDa.Observed band size : 70 kDa .
Immunohistochemistry (Paraffin-embedded sections) - HDAC2 antibody [Y461]; Immunohistochemical analysis of HDAC2 expression in paraffin embedded human breast carcinoma tissue section, using 1/250 TA300044.
Immunohistochemistry (Frozen sections) - HDAC2 antibody [Y461]; TA300044 staining HDAC2 in Rat spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at 25Â°C. Samples were incubated with primary antibody (1/500 in PBS + 0.2% TritonX + 1% BSA) for 16 hours at 4Â°C. An Alexa Fluor?488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Antigen unmasking with sodium citrate buffer (10mM sodium citrate, 0.05% Tween 20, ph6) was necessary to obtain a good signal. The sections were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence - HDAC2 antibody [Y461]; Immunofluorescent analysis of HDAC expression in HeLa cells, using 1/250 TA300044.
Flow Cytometry-HDAC2 antibody [Y461](TA300044); Overlay histogram showing HeLa cells stained with TA300044 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.