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Anti-HDAC2 Antibody Y461
Also for HDAC2 (NM_001527)
| A synthetic peptide corresponding to residues in the C-term of human Histone Deacetylase 2 was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:2000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:60 - 1:100; IP: 1:60; IHC-Fr: 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens histone deacetylase 2 (HDAC2), transcript variant 1|
|HD2; RPD3; YAF1|
|Histone deacetylase 2 (HDAC2) belongs to the histone deacetylase family type 1 which is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (1). HDAC2 represses transcription and binds transcription factor YY1 to form a large complex (2). It also forms transcriptional repressor complexes by associating with MAD, SIN3, N-CoR and DNMT1 (3-4). Similar to HDAC1 and HDAC3, HDAC2 does not possess a repression domain, but preferentially associates with HDAC1. HDAC2 with HDAC1 is also implicated in a complex of multiproteins that functions through modifying chromatin structure to keep genes silent (5). |
Delta-Notch Signaling Pathway
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Western blot - HDAC2 antibody [Y461]; Anti-HDAC2 antibody [Y461] at 1/2000 dilution + K562 cell lysate.Predicted band size : 55 kDa.Observed band size : 70 kDa .
Immunohistochemistry (Paraffin-embedded sections) - HDAC2 antibody [Y461]; Immunohistochemical analysis of HDAC2 expression in paraffin embedded human breast carcinoma tissue section, using 1/250 ab32117.
Immunohistochemistry (Frozen sections) - HDAC2 antibody [Y461]; ab32117 staining HDAC2 in Rat spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500 in PBS + 0.2% TritonX + 1% BSA) for 16 hours at 4°C. An Alexa Fluor?488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Antigen unmasking with sodium citrate buffer (10mM sodium citrate, 0.05% Tween 20, ph6) was necessary to obtain a good signal. The sections were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence - HDAC2 antibody [Y461]; Immunofluorescent analysis of HDAC expression in HeLa cells, using 1/250 ab32117.
Flow Cytometry-HDAC2 antibody [Y461](ab32117); Overlay histogram showing HeLa cells stained with ab32117 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.