Western Blot: HDAC11 Antibody - Mouse brain homogenate samples were loaded on to a 10% Prep-gel Tris-Glycine polyacrylamide gels and blotted onto PVDF membranes. The membranes was probed with 0.2ug/ml of affinity purified antibody in 5% non-fat dry milk TBST over night at 4 C. The membrane was then further probed with goat anti rabbit HRP at 1:40, 000 and developed using a standard chemiluminescence method. A major band at 39 kDa and a minor band at 37 kDa are observed. Both bands are blocked when the immunizing peptide is included in the incubation (data not shown).
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