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Home Antibody All anti-HDAC10 antibodies

Anti-HDAC10 TRUEMAB Antibody Clone OTI5E2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500787 anti-HDAC10 mouse monoclonal antibody, clone OTI5E2 (formerly 5E2) 100ul $325 In Stock
LC403142 HDAC10 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500787 Carrier-free (BSA/glycerol-free) HDAC10 mouse monoclonal antibody, clone OTI5E2 (formerly 5E2) 100ug $450 3-4 weeks
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WB(1)
IP(1)
FC(2)
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Also for HDAC10 (NM_032019)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human HDAC10 (NP_114408) produced in HEK293T cell.
Clone NameClone OTI5E2 IsotypeIgG2a
Species ReactivityHuman Concentration0.9 mg/ml
Guaranteed Application *WB, IP, FC Suggested DilutionsWB 1:2000, Flow 1:100, IP 2-4ug/mg
Predicted MW Explanation 71.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens histone deacetylase 10 (HDAC10), transcript variant 1
Alternative NameHD10
Database LinkNP_114408
Entrez Gene 83933 Human
FunctionAcetylation of histone core particles modulates chromatin structure and gene expression. The opposing enzymatic activities of histone acetyltransferases and histone deacetylases, such as HDAC10, determine the acetylation status of histone tails
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (RC218536, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.
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Immunoprecipitation of HDAC10 by using TrueMab monoclonal anti-HDAC10 antibody (Negative control: IP without adding anti-HDAC10 antibody). For each experiment, 500ul of DDK tagged HDAC10 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of
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Flow cytometric analysis of Hela cells, using anti-HDAC10 antibody(TA500787),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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Flow cytometric analysis of Jurkat cells, using anti-HDAC10 antibody(TA500787),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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