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Also for HDAC1 (NM_004964)
|This HDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the N-terminal region of human HDAC1.|
||WB: 1:1000; IF: 1:10-50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
|This antibody is purified through a protein A column, followed by peptide affinity purification.
|Homo sapiens histone deacetylase 1 (HDAC1)|
|GON-10; HD1; RPD3; RPD3L1|
Delta-Notch Signaling Pathway
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HDAC1 Antibody (N-term) western blot analysis in MCF-7 cell line and human placenta and normal uterus,rat spleen lysates (35ug/lane).This demonstrates the HDAC1 antibody detected the HDAC1 protein (arrow).
Fluorescent confocal image of MCF-7 cell stained with HDAC1 Antibody (N-term).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC1 primary antibody (1:25, 1 h at 37.). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37.).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37.). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HDAC1 immunoreactivity is localized to Nucleus significantly.