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Anti-HDAC1 Antibody EPR460(2)
Also for HDAC1 (NM_004964)
|A synthetic peptide corresponding to residues near the C-terminus of human Histone Deacetylase 1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC/IF: 1:400; WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens histone deacetylase 1 (HDAC1)|
|GON-10; HD1; RPD3; RPD3L1|
|Histone Deacetylase 1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis (1).|
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
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Western blot - HDAC1 antibody [EPR460(2)]; All lanes : Anti-HDAC1 antibody [EPR460(2)] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : K562 cell lysate.Lane 4 : MCF7 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 55 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)]; Immunohistochemical analysis of HDAC1 in paraffin-embedded Human colon tissue using TA307790 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)]; Immunohistochemical analysis of HDAC1 in paraffin-embedded Human tonsil tissue using TA307790 at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)]; TA307790 (1/400) staining HDAC1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.