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Home Antibody All anti-H3F3A antibodies

Anti-H3F3A PHOSPHO Antibody EPR1873

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307480
  • Rabbit monoclonal antibody against Histone H3.3 Phospho (pS31) (clone EPR1873 ) (Phospho-Specific)
100ul 325 3-7 Days
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WB(2)
IHC(1)
IF(1)
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Also for H3F3A (NM_002107)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding Serine 31 of human Histone H3.3 was used as an immunogen.
Clone NameEPR1873 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsChIP: Use at an assay dependent concentration; WB: 1:1000 - 1:5000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
Predicted MW Explanation 15 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens H3 histone, family 3A (H3F3A)
Alternative NameH3.3A; H3F3
Database LinkNP_002098
Entrez Gene 3020 Human
Entrez Gene 15078 Mouse
Entrez Gene 289314 Rat
FunctionChanges in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3.3 is a variant histone which replaces conventional H3 in nucleosomes in active genes. It is predominant from of histone H3 in non-dividing cells and it is incorporated into chromatin independently of DNA synthesis. H3.3 is phosphorylated at serine 28 by MLTK isoform 1, RPS6KA5, and AURKB during mitosis. Also, phosphorylation at serine 31 on H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H3.3 (phospho S31) antibody [EPR1873]; All lanes : Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - ChIP Grade at 1/1000 dilution.Lane 1 : HeLa cell lysate untreated.Lane 2 : HeLa cell lysate treated with Calyculin A.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 15 kDa.
WB Image
Western blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - ChIP Grade; .Predicted band size : 15 kDa. This image is courtesy of an anonymous customer.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3.3 (phospho S31) antibody [EPR1873]; Paraffin-embedded human cervical carcinoma labelled with TA307480 at 1/100 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H3.3 (phospho S31) antibody [EPR1873]; Immunofluorescent staining of HeLa cells using TA307480 at 1/100 dilution.

 

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