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Home Antibody All anti-H3F3A antibodies

Anti-H3F3A PHOSPHO Antibody EP1874Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307479
  • Rabbit monoclonal antibody against Histone H3.3 Phospho (pS28 & 31) (clone EP1874Y ) (Phospho-Specific)
100ul 325 3-7 Days
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WB(1)
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Also for H3F3A (NM_002107)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding serine 28 and serine 31 of human Histone H3.3 protein.
Clone NameEP1874Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:2000 - 1:5000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens H3 histone, family 3A (H3F3A)
Alternative NameH3.3A; H3F3
Database LinkNP_002098
FunctionChanges in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3.3 is a variant histone which replaces conventional H3 in nucleosomes in active genes. It is predominant from of histone H3 in non-dividing cells and it is incorporated into chromatin independently of DNA synthesis. H3.3 is phosphorylated at serine 28 by MLTK isoform 1, RPS6KA5, and AURKB during mitosis. Also, phosphorylation at serine 31 on H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Histone H3.3 (phospho S28) antibody [EP1874Y]; All lanes : Anti-Histone H3.3 (phospho S28) antibody [EP1874Y] at 1/5000 dilution.Lane 1 : HeLa cell lysates untreated.Lane 2 : HeLa cell lysates treated with FBS + calyculin A.Lysates/proteins at 10 ug per lane.Predicted band size : 15 kDa.Observed band size : 17 kDa .

 

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