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Home Antibody All anti-H2AFX antibodies

Anti-H2AFX Antibody EP854(2)Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301078
  • Rabbit Monoclonal Antibody against H2AFX (Clone EP854(2)Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400770) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(3)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding serine 139 of human H2A.x protein.
Clone NameEP854(2)Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:100000 - 1:500000; IHC-P: 1:100 - 1:5000; ICC/IF: 1:50 - 1:100; CHIPseq: Use at an assay dependent concentration; FC: 1:100
Predicted MW Explanation 15 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens H2A histone family, member X (H2AFX)
Alternative NameH2A.X; H2A/X; H2AX
Database LinkNP_002096
Entrez Gene 3014 Human
Entrez Gene 15270 Mouse
Entrez Gene 500987 Rat
FunctionAs a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair (1). As a part of post-translational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis (2)
Related PathwayDruggable Genome Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H2A.X (phospho S139) antibody [EP854(2)Y]; All lanes : Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] at 1/500000 dilution.Lane 1 : Jurkat cell lysates Jurkat cell lysates.Lane 2 : Jurkat cell lysates treated with etoposide .Lysates/proteins at 10 ug per lane.Secondary.HRP Labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 15 kDa.Observed band size : 15 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y]; IHC-P image of ?H2A.X staining onMouse testis sections using TA301078 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. TA301078 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with TA301078 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y]; IHC-P image of ?H2A.Xstaining onRattestis sections using TA301078 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. TA301078 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with TA301078 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H2A.X (phospho S139) antibody [EP854(2)Y]; TA301078, at 1/100 staining Human kidney transitional cell carcinoma by Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human kidney transitional cell carcinoma using 1/100 TA301078.
IF Image
Immunocytochemistry/ Immunofluorescence-Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y](TA301078); ICC/IF image of TA301078 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
FC Image
Flow Cytometry - Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y]; Overlay histogram showing HeLa cells stained with TA301078 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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