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Anti-H2AFX Antibody EP854(2)Y
Also for H2AFX (NM_002105)
|A synthetic phospho-peptide corresponding to residues surrounding serine 139 of human H2A.x protein.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:100000 - 1:500000; IHC-P: 1:100 - 1:5000; ICC/IF: 1:50 - 1:100; CHIPseq: Use at an assay dependent concentration; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens H2A histone family, member X (H2AFX)|
|H2A.X; H2A/X; H2AX|
Entrez Gene 3014 Human
Entrez Gene 15270 Mouse
Entrez Gene 500987 Rat
|As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair (1). As a part of post-translational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis (2) |
|Druggable Genome Systemic lupus erythematosus|
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Western blot - Histone H2A.X (phospho S139) antibody [EP854(2)Y]; All lanes : Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] at 1/500000 dilution.Lane 1 : Jurkat cell lysates Jurkat cell lysates.Lane 2 : Jurkat cell lysates treated with etoposide .Lysates/proteins at 10 ug per lane.Secondary.HRP Labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 15 kDa.Observed band size : 15 kDa.
IHC-P image of H2A.X staining on Mouse testis sections using TA301078 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. TA301078 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with TA301078 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
IHC-P image of H2A.X staining on Rattestis sections using TA301078 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. TA301078 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with TA301078 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H2A.X (phospho S139) antibody [EP854(2)Y]; TA301078, at 1/100 staining Human kidney transitional cell carcinoma by Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human kidney transitional cell carcinoma using 1/100 TA301078.
ICC/IF image of TA301078 stained HepG2 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was DyLight488 goat anti-rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry - Anti-Histone H2A.X (phospho S139) antibody; Overlay histogram showing HeLa cells stained with TA301078 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.