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Home Antibody All anti-Gdf15 antibodies

Anti-Gdf15 Antibody

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SKU Description Amount Price Availability*  
TA319471
  • Rabbit polyclonal anti-Nag-1 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401529) , 20ug Explanation
100ug 325 3-7 Days
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WB(1)

OriGene Data

ImmunogenThis affinity purified antibody was prepared by repeated immunizations with a peptide corresponding to an amino acid sequence near the C-terminal of mouse NAG-1 protein.
Clone Name IsotypeIgG
Species Reactivitymouse, human Concentration0.95 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:100,000 - 1:120,000, WB: 1:3000 - 1:7,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.

Reference Data

Target NameMus musculus growth differentiation factor 15 (Gdf15)
Alternative NameMIC-1; NAG-1; SBF
Database LinkNP_035949
Entrez Gene 9518 Human
Entrez Gene 23886 Mouse
Function
Related Pathway

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WB Image
Western blot using affinity purified anti-mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody (611-103-122) in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System. Other detection systems will yield similar results.

 

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