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Home Antibody All anti-GTF2F2 antibodies

Anti-GTF2F2 Antibody EPR7584

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SKU Description Amount Price Availability*  
TA310560
  • Rabbit Monoclonal antibody against TFIIF (GTF2F2)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418203) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(2)
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Also for GTF2F2 (NM_004128)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human TFIIF was used as an immunogen.
Clone NameEPR7584 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; ICC/IF: 1:25 - 1:50
Predicted MW Explanation 28 kDa
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2)
Alternative NameBTF4; RAP30; TF2F2; TFIIF
Database LinkNP_004119
Entrez Gene 2963 Human
Entrez Gene 68705 Mouse
Entrez Gene 81674 Rat
FunctionTFIIF functions in both transcription initiation and elongation and is one of three general factors, TFIIB, TFIIE, and TFIIF, that directly binds RNA polymerase II (Pol II). TFIIF facilitates Pol II incorporation into a PIC intermediate-containing promoter DNA, TBP, and TFIIB and is required for subsequent recruitment of TFIIE and TFIIH. TFIIF also has functions in open complex formation, transcription start site selection, promoter escape, stabilization of an RNA–DNA hybrid within Pol II, and, along with TFIIS, stimulates elongation by purified Pol II (1).
Related PathwayTranscription Factors Basal transcription factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-GTF2F2 antibody [EPR7584]; All lanes : Anti-GTF2F2 antibody [EPR7584] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 28 kDa.Observed band size : 30 kDa .
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584]; TA310560 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584]; TA310560 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human colon tissue by immunohistochemistry.
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