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Home Antibody All anti-GTF2F2 antibodies

Anti-GTF2F2 Antibody EPR7584

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310560
  • Rabbit Monoclonal antibody against TFIIF (GTF2F2)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC418203) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(2)
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Also for GTF2F2 (NM_004128)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human TFIIF was used as an immunogen.
Clone NameEPR7584 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; ICC/IF: 1:25 - 1:50
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2)
Alternative NameBTF4; RAP30; TF2F2; TFIIF
Database LinkNP_004119
FunctionTFIIF functions in both transcription initiation and elongation and is one of three general factors, TFIIB, TFIIE, and TFIIF, that directly binds RNA polymerase II (Pol II). TFIIF facilitates Pol II incorporation into a PIC intermediate-containing promoter DNA, TBP, and TFIIB and is required for subsequent recruitment of TFIIE and TFIIH. TFIIF also has functions in open complex formation, transcription start site selection, promoter escape, stabilization of an RNA–DNA hybrid within Pol II, and, along with TFIIS, stimulates elongation by purified Pol II (1).
Related Pathway

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WB Image
Western blot - Anti-GTF2F2 antibody [EPR7584]; All lanes : Anti-GTF2F2 antibody [EPR7584] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 28 kDa.Observed band size : 30 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584]; TA310560 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584]; TA310560 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human colon tissue by immunohistochemistry.

 

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