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Anti-GSN Antibody EPR1942
|A synthetic peptide corresponding to residues in human Gelsolin was used as an immunogen.|
||Tissue culture supernatant
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:50000 - 1:20000; IHC-P: 1:250 - 1:500; FC: 1:50 - 1:1000; ICC/IF: 1:100 - 1:250
|Does not react with Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for IP.
|Homo sapiens gelsolin (GSN), transcript variant 1|
|Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).|
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Western blot - Gelsolin antibody [EPR1942]; All lanes : Anti-Gelsolin antibody [EPR1942] at 1/50000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : HL60 cell lysate.Lane 3 : A431 cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 86 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gelsolin antibody [EPR1942]; Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human kidney tissue using TA307746 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gelsolin antibody [EPR1942]; Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human lung tissue using TA307746 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Gelsolin antibody [EPR1942]; Immunofluorescent staining of Gelsolin in HeLa cells using TA307746 at 1/100 dilution.
Flow Cytometry - Anti-Gelsolin antibody [EPR1942]; Overlay histogram showing HL60 cells stained with TA307746 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.