OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-GSN antibodies

Anti-GSN Antibody EPR1942

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307746 Rabbit monoclonal antibody against Gelsolin(clone EPR1942) 100ul $325 In Stock
LC424875 GSN HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
Add to Shopping Cart

spacer
WB(1)
IHC(2)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Gelsolin was used as an immunogen.
Clone NameEPR1942 IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:50000 - 1:20000; IHC-P: 1:250 - 1:500; FC: 1:50 - 1:1000; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens gelsolin (GSN), transcript variant 1
Alternative NameADF; AGEL
Database LinkNP_000168
Entrez Gene 2934 Human
Entrez Gene 227753 Mouse
FunctionGelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).
Related PathwaySecreted ProteinDruggable Genome Fc gamma R-mediated phagocytosisRegulation of actin cytoskeleton

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Gelsolin antibody [EPR1942]; All lanes : Anti-Gelsolin antibody [EPR1942] at 1/50000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : HL60 cell lysate.Lane 3 : A431 cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 86 kDa.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gelsolin antibody [EPR1942]; Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human kidney tissue using TA307746 at 1/250 dilution.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gelsolin antibody [EPR1942]; Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human lung tissue using TA307746 at 1/250 dilution.
spacer
Immunocytochemistry/ Immunofluorescence - Gelsolin antibody [EPR1942]; Immunofluorescent staining of Gelsolin in HeLa cells using TA307746 at 1/100 dilution.
spacer
Flow Cytometry - Anti-Gelsolin antibody; Overlay histogram showing HL60 cells stained with TA307746 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in HL60 cells under the same conditions.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title