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Home Antibody All anti-GRM1 antibodies

Anti-GRM1 Antibody EP691Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300658
  • Rabbit Monoclonal Antibody against Chk1 (CHEK1, C-terminus)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400510) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to C-terminal residues of human Chk1 was used as an immunogen.
Clone NameEP691Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:10000; FC: 1:1000; IHC-P: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3
Alternative NameCHK1
Database LinkNP_001265
FunctionChk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).
Related Pathway
Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Chk1 antibody [EP691Y]; Anti-Chk1 antibody [EP691Y] at 1/10000 dilution + HeLa cell lysate at 10 ug.Predicted band size : 54 kDa.Observed band size : 54 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Chk1 antibody [EP691Y]; Immunohistochemical analysis of Chk1 expression in paraffin-embedded human breast carcinoma using TA300658 at a 1:250 dilution.
FC Image
Flow Cytometry - Anti-Chk1 antibody [EP691Y]; Overlay histogram showing HeLa cells stained with TA300658 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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