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Anti-GRB2 Antibody Y301
Also for GRB2 (NM_002086)
|A synthetic peptide corresponding to residues in the N-terminus of human GRB2 was used as an immunogen. This antibody can also detect the splice isoform GRB3-3 of GRB2 according to sequence analysis.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:2000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; FC: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens growth factor receptor-bound protein 2 (GRB2), transcript variant 1|
|ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; NCKAP2|
|The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3). |
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
MAPK signaling pathway
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Western blot - GRB2 antibody [Y301]; All lanes : Anti-GRB2 antibody [Y301] at 1/2000 dilution.Lane 1 : NIH 3T3 cell lysate.Lane 2 : PC12 cell lysate.Lane 3 : HeLa cell lysate.Predicted band size : 25 kDa.Observed band size : 25 kDa.
Immunohistochemistry (Paraffin-embedded sections) - GRB2 antibody [Y301]; Immunohistochemical analysis of GRB2 expression in paraffin embedded human breast carcinoma tissue section, using 1/100 ab32111.
Immunocytochemistry/ Immunofluorescence - GRB2 antibody [Y301]; Immunofluorescent analysis of GRB2 expression in PC12 cells, using 1/100 ab32111.
Flow Cytometry-Anti-GRB2 antibody [Y301](ab32111); Overlay histogram showing SH-SY5Y cells stained with ab32111 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.