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Anti-GPX1 Antibody EPR3312
Also for GPX1 (NM_000581)
|A synthetic peptide corresponding to residues in human GPX1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:100 - 1:1000; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens glutathione peroxidase 1 (GPX1), transcript variant 1|
|GPX1 is a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and it is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase. This active site is coded by UGA, normally functioning as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk.|
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Western blot - Glutathione Peroxidase 1 antibody [EPR3312]; All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3312] at 1/1000 dilution.Lane 1 : Human fetal liver lysate.Lane 2 : 293T cell lysate.Lane 3 : THP1 cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 22 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glutathione Peroxidase 1 antibody [EPR3312]; ab108427, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Glutathione Peroxidase 1 antibody [EPR3312]; ab108427, at 1/100 dilution, staining Glutathione Peroxidase 1 in 293T cells by Immunofluorescence.
Flow Cytometry - Anti-Glutathione Peroxidase 1 antibody [EPR3312]; Overlay histogram showing HepG2 cells stained with ab108427 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.