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Home Antibody All anti-GPX1 antibodies

Anti-GPX1 Antibody EPR3312

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307689
  • Rabbit monoclonal antibody against GPX1(clone EPR3312)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424631) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human GPX1 was used as an immunogen.
Clone NameEPR3312 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:100 - 1:1000; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 22 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens glutathione peroxidase 1 (GPX1), transcript variant 1
Alternative NameGPXD; GSHPX1
Database LinkNP_000572
Entrez Gene 2876 Human
Entrez Gene 14775 Mouse
Entrez Gene 24404 Rat
FunctionGPX1 is a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and it is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase. This active site is coded by UGA, normally functioning as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk.
Related PathwayDruggable Genome Glutathione metabolismArachidonic acid metabolismAmyotrophic lateral sclerosis (ALS)Huntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Glutathione Peroxidase 1 antibody [EPR3312]; All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3312] at 1/1000 dilution.Lane 1 : Human fetal liver lysate.Lane 2 : 293T cell lysate.Lane 3 : THP1 cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 22 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glutathione Peroxidase 1 antibody [EPR3312]; TA307689, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Glutathione Peroxidase 1 antibody [EPR3312]; TA307689, at 1/100 dilution, staining Glutathione Peroxidase 1 in 293T cells by Immunofluorescence.
FC Image
Flow Cytometry - Anti-Glutathione Peroxidase 1 antibody [EPR3312]; Overlay histogram showing HepG2 cells stained with TA307689 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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