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Anti-GPI TRUEMAB Antibody clone 5A11
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full length human recombinant protein of human GPI(NP_000166) produced in HEK293T cell.|
|Human , Dog , Monkey
|WB, IHC, IF, FC
||WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens glucose-6-phosphate isomerase (GPI), transcript variant 2|
|AMF; GNPI; NLK; PGI; PHI; SA-36; SA36|
|This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPI (RC201232, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPI .
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPI monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501137, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501137, Dilution 1:50)
Anti-GPI mouse monoclonal antibody (TA501137) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPI (RC201232).
Flow cytometric Analysis of Jurkat cells, using anti-GPI antibody(TA501137),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).