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Home Antibody All anti-GPI antibodies

Anti-GPI TRUEMAB Antibody clone 5A11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501137
  • anti-GPI (Glucose 6 phosphate isomerase) mouse monoclonal antibody, clone 5A11
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400066) , 20ug Explanation
100ul 379 In Stock
CF501137
  • Carrier-free (BSA/glycerol-free) GPI mouse monoclonal antibody, clone 5A11
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(2)
IF(1)
FC(1)
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Also for GPI (NM_000175)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human GPI(NP_000166) produced in HEK293T cell.
Clone Nameclone 5A11 IsotypeIgG2b
Species ReactivityHuman , Dog , Monkey Concentration0.72 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Predicted MW Explanation 63.0 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens glucose-6-phosphate isomerase (GPI), transcript variant 2
Alternative NameAMF; GNPI; NLK; PGI; PHI; SA-36; SA36
Database LinkNP_000166
Entrez Gene 2821 Human
Entrez Gene 717933 Monkey
Entrez Gene 611942 Dog
FunctionThis gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Related PathwayDruggable Genome Glycolysis / GluconeogenesisPentose phosphate pathwayStarch and sucrose metabolismAmino sugar and nucleotide sugar metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPI (RC201232, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPI .
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPI monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501137, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501137, Dilution 1:50)
IF Image
Anti-GPI mouse monoclonal antibody (TA501137) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPI (RC201232).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-GPI antibody(TA501137),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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