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Anti-GPI TRUEMAB Antibody Clone OTI8G7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501135 anti-GPI (Glucose 6 phosphate isomerase) mouse monoclonal antibody, clone OTI8G7 (formerly 8G7) 100ul 325
In Stock
LC400066 GPI HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501135 Carrier-free (BSA/glycerol-free) GPI mouse monoclonal antibody, clone OTI8G7 (formerly 8G7) 100ug $450 3-4 weeks
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OriGene Data

ImmunogenFull length human recombinant protein of human GPI(NP_000166) produced in HEK293T cell.
Clone NameClone OTI8G7 IsotypeIgG2b
Species ReactivityHuman Concentration0.75 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Predicted MW Explanation 63kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens glucose-6-phosphate isomerase (GPI), transcript variant 2
Alternative NameAMF; GNPI; NLK; PGI; PHI; SA-36; SA36
Database LinkNP_000166
Entrez Gene 2821 Human
FunctionThis gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Related PathwayDruggable Genome Glycolysis / GluconeogenesisPentose phosphate pathwayStarch and sucrose metabolismAmino sugar and nucleotide sugar metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPI (RC201232, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPI.
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501135, Dilution 1:50)
Anti-GPI mouse monoclonal antibody (TA501135) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPI(RC201232).
Immunofluorescent staining of HT29 cells using anti-GPI mouse monoclonal antibody (TA501135).
HEK293T cells transfected with either pCMV6-ENTRY GPI(RC201232)(Red) or empty vector control plasmid(Blue) were immunostained with anti-GPI mouse monoclonal(TA501135), and then analyzed by flow cytometry.


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