Anti-GPHN Antibody, clone 3B6

Also for GPHN (NM_020806)

OriGene Data

Immunogen	Full length human recombinant protein of human GPHN (NP_065857) produced in HEK293T cell.
Clone Name	clone 3B6	Isotype	IgG2a
Species Reactivity	Human, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens gephyrin (GPHN), transcript variant 1
Alternative Name	GEPH; GPH; GPHRYN; HKPX1
Database Link	NP_065857
Function	This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPHN monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GPHN mouse monoclonal antibody. (TA502217)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-GPHN mouse monoclonal antibody. (TA502217)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GPHN mouse monoclonal antibody. (TA502217)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-GPHN mouse monoclonal antibody. (TA502217)

IF Image

Anti-GPHN mouse monoclonal antibody (TA502217) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPHN(RC205986).

FC Image

HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502217), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-GPHN antibody(TA502217),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-GPHN TrueMAB Antibody clone 3B6

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OriGene Data

Reference Data