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Anti-GORASP1 TRUEMAB Antibody Clone OTI5C5
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for GORASP1 (NM_031899)
|Full length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell.|
|Human , Dog , Rat
|WB, IHC, IF, FC
||WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens golgi reassembly stacking protein 1, 65kDa (GORASP1), transcript variant 1|
|GOLPH5; GRASP65; P65|
Entrez Gene 64689 Human
Entrez Gene 56082 Rat
Entrez Gene 485594 Dog
|The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GORASP1 (RC210076, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GORASP1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GORASP1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GORASP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501352, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GORASP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501352, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GORASP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501352, Dilution 1:50)
Anti-GORASP1 mouse monoclonal antibody (TA501352) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GORASP1(RC210076).
HEK293T cells transfected with either RC210076 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GORASP1 antibody(TA501352), and then analyzed by flow cytometry.