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Anti-GLUL TRUEMAB Antibody Clone OTI1F4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500700 GLUL mouse monoclonal antibody, clone OTI1F4 (formerly 1F4) 100ul
30ul
In Stock
LC400756 GLUL HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500700 Carrier-free (BSA/glycerol-free) GLUL mouse monoclonal antibody, clone OTI1F4 (formerly 1F4) 100ug $450 3-4 weeks
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WB(2)
IHC(3)
IF(2)
FC(2)

OriGene Data

ImmunogenFull length human recombinant protein of human GLUL (NP_002056) produced in HEK293T cell.
Clone NameClone OTI1F4 IsotypeIgG2a
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:50~100, FLOW 1:100,
Predicted MW Explanation 41.9 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens glutamate-ammonia ligase (GLUL), transcript variant 1
Alternative NameGLNS; GS; PIG43; PIG59
Database LinkNP_002056
Entrez Gene 2752 Human
FunctionThe protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. This gene is expressed during early fetal stages, and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in this gene are associated with congenital glutamine deficiency. Several alternatively spliced transcript variants have been found for this gene.
Related Pathway Alanine, aspartate and glutamate metabolismArginine and proline metabolismNitrogen metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLUL (RC204161, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLUL.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-GLUL monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GLUL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500700)
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GLUL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500700)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GLUL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500700)
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Anti-GLUL mouse monoclonal antibody (TA500700) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GLUL(RC204161).
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Immunofluorescent staining of HepG2 cells using anti-GLUL mouse monoclonal antibody (TA500700).
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Flow cytometric Analysis of Hela cells, using anti-GLUL antibody(TA500700),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-GLUL antibody(TA500700),(Red), compared to a nonspecific negative control antibody,(Blue).
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