Anti-GLI1 Antibody, EPR4523

Also for GLI1 (NM_001160045)

OriGene Data

Immunogen	A synthetic peptide corresponding to residues in human Gli-1 was used as an immunogen.
Clone Name	EPR4523	Isotype
Species Reactivity	Human	Concentration
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB: 1:1,000 - 10,000;
Buffer	Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant 2
Alternative Name	GLI
Database Link	NP_001153517
Function	Gli-1 is a member of the Kruppel family of zinc finger proteins. This transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop (1).
Related Pathway	Hedgehog signaling pathway

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WB Image

Western blot analysis on (A) RMS-13 and (B) A673 cell lysates using anti-Gli-1 RabMAb. Note: RMS-13 lysate was kindly provided by Martin Fernandez-Zapico at Mayo Clinic.

IHC Image

Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Gli1 RabMAb.

IHC Image

Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-Gli1 RabMAb.

IF Image

Western blot analysis on (A) RMS-13 and (B) A673 cell lysates using anti-Gli-1 RabMAb. Note: RMS-13 lysate was kindly provided by Martin Fernandez-Zapico at Mayo Clinic.

IF Image

Immunofluorescent staining of A673 cells using anti-Gli1 RabMAb.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
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DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-GLI1 Antibody EPR4523

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data