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Home Antibody All anti-GK antibodies

Anti-GK Antibody EPR6567

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310534
  • Rabbit Monoclonal antibody against Glycerol Kinase (GK)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC404332) , 20ug
100ul 325 3-7 Days
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WB(2)
FC(1)
Assay(1)
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Also for GK (NM_203391)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Glycerol Kinase was used as an immunogen.
Clone NameEPR6567 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100 - 1:500
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for ICC or IHC-P.

Reference Data

Target NameHomo sapiens glycerol kinase (GK), transcript variant 1
Alternative NameGK1; GKD
Database LinkNP_976325
FunctionGlycerol Kinase belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in Glycerol Kinase are associated with glycerol kinase deficiency (GKD) (1).
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Glycerol kinase antibody [EPR6567]; All lanes : Anti-Glycerol kinase antibody [EPR6567] at 1/1000 dilution.Lane 1 : Mouse adipose tissue lysate.Lane 2 : Mouse adipose tissue lysate.Lysates/proteins at 25 µg per lane.Secondary.HRP-conjugated Donkey anti-rabbit polyclonal at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 61 kDa.Observed band size : 55 kDa .Exposure time : 5 minutesThis image is courtesy of an anonymous Abreview
WB Image
Western blot - Anti-Glycerol kinase antibody [EPR6567]; All lanes : Anti-Glycerol kinase antibody [EPR6567] at 1/1000 dilution.Lane 1 : Fetal liver lysate.Lane 2 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 61 kDa.Observed band size : 55 kDa .
Assay Image
Other-Anti-Glycerol kinase antibody [EPR6567](TA310534); Equilibrium disassociation constant (KD)..
FC Image
Flow Cytometry - Anti-Glycerol kinase antibody [EPR6567]; TA310534, at 1/100 dilution, staining Glycerol kinase in permeabilized HepG2 cells by Immunofluorescence (red). A rabbit IgG is used as a negative control (green).

 

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