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Anti-GFPT1 Antibody EPR4854


Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
  • Rabbit Monoclonal antibody against GFAT1
100ul 325 3-7 Days
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Also for GFPT1 (NM_002056)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human GFAT1 was used as an immunogen.
Clone NameEPR4854 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250
Predicted MW Explanation 79 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or ICC.

Reference Data

Target NameHomo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 2
Database LinkNP_002047
Entrez Gene 2673 Human
Entrez Gene 14583 Mouse
Entrez Gene 297417 Rat
FunctionGFAT1 is the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. It catalyzes the formation of glucosamine 6-phosphate (1). The GFAT-mediated amino sugar pathway has been shown to be involved in the desensitization of adipocytes to insulin by glucose in non-insulin-dependent diabetes mellitus (2). It is most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins (3).
Related PathwayProtease Alanine, aspartate and glutamate metabolismAmino sugar and nucleotide sugar metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-GFPT1 antibody [EPR4854]; All lanes : Anti-GFPT1 antibody [EPR4854] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : JAR cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : Human placenta lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.developed using the ECL technique.Predicted band size : 79 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFPT1 antibody [EPR4854]; TA310529, at a 1/100 dilution, staining GFPT1 in paraffin-embedded Human testis tissue by immunohistochemistry.


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