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Home Antibody All anti-GCLM antibodies

Anti-GCLM Antibody EPR6667

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA310523
  • Rabbit Monoclonal antibody against GCLM
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400752) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human GCLM was used as an immunogen.
Clone NameEPR6667 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; FC: 1:10 - 1:100
Predicted MW Explanation 31 kDa
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for ICC.

Reference Data

Target NameHomo sapiens glutamate-cysteine ligase, modifier subunit (GCLM)
Alternative NameGLCLR
Database LinkNP_002052
Entrez Gene 2730 Human
Entrez Gene 14630 Mouse
Entrez Gene 29739 Rat
FunctionGCLM (Glutamate-cysteine ligase) is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLM deficiency has been implicated in some forms of hemolytic anemia (1).
Related PathwayDruggable Genome Glutathione metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-GCLM antibody [EPR6667]; All lanes : Anti-GCLM antibody [EPR6667] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : A673 cell lysate.Lane 3 : PC12 cell lysate.Lane 4 : A431 cell lysate.Lane 5 : K562 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 31 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667]; TA310523, at 1/50 dilution, staining GCLM in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
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Flow Cytometry - Anti-GCLM antibody [EPR6667]; Overlay histogram showing HeLa cells stained with TA310523 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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