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Home Antibody All anti-GCLM antibodies

Anti-GCLM Antibody EPR6668

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310522
  • Rabbit Monoclonal antibody against GCLM
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400752) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
Assay(1)
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Also for GCLM (NM_002061)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human GCLM was used as an immunogen.
Clone NameEPR6668 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:100 - 1:500; ICC/IF: 1:250 - 1:500
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens glutamate-cysteine ligase, modifier subunit (GCLM)
Alternative NameGLCLR
Database LinkNP_002052
Entrez Gene 2730 Human
Entrez Gene 14630 Mouse
Entrez Gene 29739 Rat
FunctionGCLM (Glutamate-cysteine ligase) is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLM deficiency has been implicated in some forms of hemolytic anemia (1).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-GCLM antibody [EPR6668]; All lanes : Anti-GCLM antibody [EPR6668] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : A673 cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP conjugated goat anti-rabbit at 1/2000 dilution.Predicted band size : 31 kDa.Observed band size : 30 kDa .
Assay Image
Other-Anti-GCLM antibody [EPR6668](TA310522); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6668]; TA310522, at 1/100 dilution, staining GCLM in paraffin-embedded Human muscle tissue by Immunohistochemistry.

 

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