Anti-GBE1 Antibody, Clone 1D11

Also for GBE1 (NM_000158)

OriGene Data

Immunogen	Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.
Clone Name	Clone 1D11	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, IP, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:50, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens glucan (1,4-alpha-), branching enzyme 1 (GBE1)
Alternative Name	APBD; GBE; GSD4
Database Link	NP_000149
Function	The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq].
Related Pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-GBE1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC Image

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-GBE1 mouse monoclonal antibody. (TA500829)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-GBE1 mouse monoclonal antibody. (TA500829)

IHC Image

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-GBE1 mouse monoclonal antibody. (TA500829)

IF Image

Anti-GBE1 mouse monoclonal antibody (TA500829) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1(RC204152).

IF Image

Immunofluorescent staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody (TA500829).

IP Image

Immunoprecipitation(IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image

HEK293T cells transfected with either RC204152 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GBE1 antibody(TA500829), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-GBE1 antibody(TA500829),(Red), compared to a nonspecific negative control antibody,(Blue).

Anti-GBE1 TrueMAB Antibody Clone 1D11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data