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Anti-GBA Antibody EPR5142
|A synthetic peptide corresponding to residues in human Glucosylceramidase was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC or IP.
|Homo sapiens glucosidase, beta, acid (GBA), transcript variant 1|
|GBA1; GCB; GLUC|
|Glucosylceramidase is a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in Glucosylceramidase cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides (1). |
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Western blot - Anti-GBA antibody [EPR5142]; All lanes : Anti-GBA antibody [EPR5142] at 1/1000 dilution.Lane 1 : Saos-2 cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : SH-SY5Y cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.developed using the ECL technique.Predicted band size : 60 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBA antibody [EPR5142]; ab125065, at a 1/50 dilution, staining GBA in paraffin-embedded Human colon tissue by immunohistochemistry.