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Anti-GAST GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for GAST (NM_000805)
|DNA immunization. This antibody is specific for the entire range of the target protein.|
||WB 1:20000, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens gastrin (GAST)|
Entrez Gene 2520 Human
|Gastrin is mainly known by its first described role as a gastric acid-hormone but the protein also has the ability to promote cellular growth. More recently, gastrin has been suggested to induce leukocyte-endothelial cell interactions and to have a pro-inflammatory effect. Gastrin has two biologically active peptide forms, G34 and G17 and they activate two different receptors: the CCK-1 receptor, which has low affinity for gastrin but high affinity for the related hormone cholecystokinin (CCK), and the CCK-2 receptor, which has high affinity for both gastrin and CCK and mediates the acid-secretory as well as the proliferative effects of gastrin.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAST (RC210222, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAST.