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Home Antibody All anti-GAPDH antibodies

Anti-GAPDH Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA308884
  • Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419567) , 20ug Explanation
100ul 325 In Stock
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WB(6)
IHC(1)
IF(1)
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Also for GAPDH (NM_002046)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 1 and 335 of GAPDH (Uniprot ID#P04406)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Cat, Chicken, Dog, Drosophila, Monkey, Pig, Rabbit, Xenopus laevis, Zebrafish, Bovine) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:5000-1:20000
Predicted MW Explanation 36kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Cat (96%), Chicken (92%), Dog (95%), Monkey (99%), Pig (95%), Rabbit (94%), Xenopus laevis (85%), Zebrafish (86%), Bovine (94%)

Reference Data

Target NameHomo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 1
Alternative NameG3PD; GAPD; HEL-S-162eP
Database LinkNP_002037
Entrez Gene 2597 Human
Entrez Gene 14433 Mouse
Entrez Gene 716991 Monkey
Entrez Gene 403755 Dog
FunctionThe product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
Related PathwayES Cell Differentiation/IPS Glycolysis / GluconeogenesisMetabolic pathwaysAlzheimer's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
GAPDH antibody detects GAPDH protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug NIH-3T3 whole cell lysate/extract. C. 30 ug mouse brain lysate/extract. D. 30 ug PC-12 whole cell lysate/extract. E. 30 ug rat brain lysate/ex
WB Image
GAPDH antibody detects GAPDH protein by Western blot analysis. A. 0.1 ug 293T whole cell lysate/extract. B. 1 ug 293T whole cell lysate/extract. C. 10 ug 293T whole cell lysate/extract. 12 % SDS-PAGE. GAPDH antibody (TA308884) dilution: 1:10000
WB Image
GAPDH antibody detects GAPDH protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract |. D. 30 ug HepG2 whole cell lysate/extract. 10 % SDS-PAGE. GAPDH anti
WB Image
GAPDH antibody detects GAPDH protein by western blot analysis. A. 20 ug 293T whole cell lysate/extract. B. 10 ug 293T whole cell lysate/extract. C. 5 ug 293T whole cell lysate/extract. D. 1 ug 293T whole cell lysate/extract. 10 % SDS-PAGE. GAPDH antibody
WB Image
GAPDH antibody detects GAPDH protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole
WB Image
GAPDH antibody detects GAPDH protein by western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 10 % SDS-PAGE. GAPDH antibody (TA308884) dilution: 1:10000
IHC Image
Immunohistochemical analysis of Salivary gland cancer, using GAPDH(TA308884) antibody at 1:500 dilution.
IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH(TA308884) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (red) at 1:2000.

 

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