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Anti-GAPDH Antibody, Loading Control
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Also for GAPDH (NM_002046)
| Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.|
|Test: Human, Mouse, Rat. Expected from seq similarity: Human, Mouse, Dog
||Lot dependent; please refer to CoA along with shipment
||WB: 0.01 - 0.03ug/ml, IHC: 0.3ug/ml, ICC: 0.1 - 0.3ug/ml, ELISA 1:2000
|Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.|
| Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
|In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed
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TA302944 staining (0.01µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302944 (0.1µg/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated GAPDH knock-down expresson in right lane) (35µg protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.
TA302944 (0.1ug/ml) staining of PFA-fixed HeLa before (top) and after (bottom) si-RNA-mediated GAPDH knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain (right).