Anti-GAPDH Antibody,

Also for GAPDH (NM_002046)

OriGene Data

Immunogen	Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
Clone Name		Isotype	Goat IgG
Species Reactivity	Human	Concentration	0.5 mg/ml
Guaranteed Application *	WB, IHC	Suggested Dilutions	ELISA: 1:2,000. WB: 0.01-0.03µg/ml. IHC 0.3ug/ml.
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 1
Alternative Name	G3PD; GAPD
Database Link	NP_002037
Function	The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

TA302944 staining (0.01µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

IHC Image

TA302944dded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Staining of germinal centre cells. B) Staining of endothelial cells.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-GAPDH Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data