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Home Antibody All anti-GAD1 antibodies

Anti-GAD1 TRUEMAB Antibody clone 5D8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500330
  • anti-GAD1 (GAD67) mouse monoclonal antibody, clone 5D8
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400290) , 20ug Explanation
100ul 325 In Stock
CF500330
  • Carrier-free (BSA/glycerol-free) GAD1 mouse monoclonal antibody,clone 5D8
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(1)
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Also for GAD1 (NM_000817)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human GAD1 expression vector
Clone Nameclone 5D8 IsotypeIgG1
Species ReactivityHuman Concentration0.5 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB 1:1000, IHC 1:50
Predicted MW Explanation 66.9 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67
Alternative NameCPSQ1; GAD; SCP
Database LinkNP_000808
Entrez Gene 2571 Human
FunctionThis gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
Related PathwayDruggable Genome Alanine, aspartate and glutamate metabolismbeta-Alanine metabolismTaurine and hypotaurine metabolismButanoate metabolismMetabolic pathwaysType I diabetes mellitusMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1 (RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1.
IHC Image
Immunohistochemical staining of paraffin-embedded breast tissue using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500330, Dilution 1:50)

 

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