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Anti-GAD1 TRUEMAB Antibody clone 5D8
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for GAD1 (NM_000817)
|Full-length protein expressed in 293T cell transfected with human GAD1 expression vector|
|WB, IHC, IP
||WB: 1:200 - 1:1000, IHC: 1:50, IP: 4ug/mL
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1 (RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1.
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-GAD1 monoclonal antibody.(1:200)
Immunohistochemical staining of paraffin-embedded breast tissue using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500330, Dilution 1:50)
Immunoprecipitation of GAD1 by using TrueMab monoclonal anti-GAD1 antibodies (Negative control: IP without adding anti-GAD1 antibody.). For each experiment, 500ul of DDK tagged GAD1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GAD1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.