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Home Antibody All anti-GAD1 antibodies

Anti-GAD1 Antibody

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SKU Description Amount Price Availability*  
TA308881 Rabbit Polyclonal antibody to GAD67 (glutamate decarboxylase 1 (brain, 67kDa)) 100ul $325 2 Weeks
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WB(4)
IHC(1)
IF(1)

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 5 and 216 of GAD67 (Uniprot ID#Q99259)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat (Predicted: Chimpanzee) Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
Predicted MW Explanation 67kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Chimpanzee (100%)

Reference Data

Target NameHomo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD25
Alternative NameCPSQ1; GAD; SCP
Database LinkNP_038473
Entrez Gene 2571 Human
Entrez Gene 14415 Mouse
Entrez Gene 24379 Rat
FunctionThis gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]
Related PathwayDruggable Genome Alanine, aspartate and glutamate metabolismbeta-Alanine metabolismTaurine and hypotaurine metabolismButanoate metabolismMetabolic pathwaysType I diabetes mellitusMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

GAD67 antibody detects GAD67 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 7.5 % SDS-PAGE. GAD67 antibody (TA308881) dilution: 1:500
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GAD67 antibody detects GAD67 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human GAD1-transfected 293T cells. 7.5 % SDS-PAGE. GAD67 antibody (TA308881) dilution: 1:5000
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GAD67 antibody detects GAD67 protein by Western blot analysis. A. 30 ug SK-N-SH whole cell lysate/extract. B. 30 ug SK-N-AS whole cell lysate/extract. 7.5 % SDS-PAGE. GAD67 antibody (TA308881) dilution: 1:500
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GAD67 antibody detects GAD67 protein by Western blot analysis. A. 50 ug mouse brian lysate/extract. 7.5 % SDS-PAGE. GAD67 antibody (TA308881) dilution: 1:500
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GAD67 antibody detects GAD67 protein at on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. GAD67 antibody (TA308881) dilution: 1:500.
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GAD67 antibody detects GAD67 protein by immunofluorescent analysis. @Sample: GAD67-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min.
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