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Home Antibody All anti-GABBR2 antibodies

Anti-GABBR2 Antibody EP2411Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303867
  • Rabbit monoclonal antibody against GABA(B) R2 (EP2411Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417297) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for GABBR2 (NM_005458)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human GABA(B) R2 was used as an immunogen.
Clone NameEP2411Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:500; IHC: 1:100; ICC: 1:50; FC: 1:40
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens gamma-aminobutyric acid (GABA) B receptor, 2 (GABBR2)
Alternative NameGABABR2; GPR51; GPRC3B; HG20; HRIHFB2099
Database LinkNP_005449
Entrez Gene 9568 Human
Entrez Gene 242425 Mouse
Entrez Gene 83633 Rat
FunctionB-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase. See GABBR1 (MIM 603540) for additional background information on GABA-B receptors.[supplied by OMIM].
Related Pathway
Signaling by GPCR

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - GABA B Receptor 2 antibody [EP2411Y]; Anti-GABA B Receptor 2 antibody [EP2411Y] at 1/500 dilution + human cerebellum lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 106 kDa.Observed band size : 106 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GABA B Receptor 2 antibody [EP2411Y]; TA303867, at a 1/100 dilution, staining GABA B Receptor 2 in paraffin embedded human brain tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 2 antibody [EP2411Y]; Immunofluorescence analysis of GABA B Receptor 2 using TA303867 at 1/400 dilution. Left panel = normal rat DRG neurons. .Right panel = rat DRG neurons transfected with siRNAs to disrupt GABA B Receptor 2.
FC Image
Flow Cytometry-Anti-GABA B Receptor 2 antibody [EP2411Y](TA303867); Overlay histogram showing SH-SY5Y cells stained with TA303867 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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