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Home Antibody All anti-FRAP1 antibodies

Anti-FRAP1 Antibody Y391


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300537 Rabbit Monoclonal Antibody against FRAP1 (Clone Y391) 100ul $325 In Stock
LC417627 MTOR HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for FRAP1 (NM_004958)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-term of PI3K/PI4K domain of human mTOR/FRAP was used as an immunogen
Clone NameY391 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsIHC-P: Use at an assay dependent dilution; WB: 1:1000 - 1:2000; ICC/IF: 1:50 - 1:100; FC: 1:50 - 1:80; IP: 1:50
Predicted MW Explanation 289 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens mechanistic target of rapamycin (serine/threonine kinase) (MTOR)
Alternative NameFRAP; FRAP1; FRAP2; RAFT1; RAPT1
Database LinkNP_004949
Entrez Gene 2475 Human
Entrez Gene 56717 Mouse
Entrez Gene 56718 Rat
FunctionThe mammalian target of rapamycin (mTOR) is a major effector of cell growth and proliferation via the regulation of protein synthesis. mTOR is composed of an N-term; 20 tandem repeats- HEAT which are implicated in protein-protein interactions and a C-term; which includes a FAT domain, a FBR domain, a kinase domain, a NDR domain and a FATC domain. The FATC domain is essential to mTOR activity and the deletion of a single amino acid from this domain abrogates the activity. mTOR can be auto-phosphorylated via its intrinsic serine/threonine kinase activity. mTOR regulates protein synthesis through the phosphorylation and inactivation of the repressor of mRNA translation 4E-BP1 and though the phosphorylation and activation of S6 kinase. RAPTOR (regulatory associated protein of TOR) is a positive regulator of TOR. Other known mediators of mTOR include PI3-K and ATK from the insulin pathway.
Related PathwayProtein KinaseDruggable Genome ErbB signaling pathwaymTOR signaling pathwayInsulin signaling pathwayAdipocytokine signaling pathwayType II diabetes mellitusPathways in cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - mTOR antibody [Y391]; All lanes : Anti-mTOR antibody [Y391] - ChIP Grade at 1/2000 dilution.Lane 1 : Hela cell lysate.Lane 2 : MCF-7.Predicted band size : 289 kDa.Observed band size : >250 kDa .

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - mTOR antibody [Y391]; Immunohistochemical analysis of mTOR expression in paraffin-embedded human prostate carcinoma, using 1/250 TA300537.

IF Image
ICC/IF image of TA300537 stained MCF7 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

FC Image
Flow Cytometry-mTOR antibody- ChIP Grade(TA300537); Overlay histogram showing HeLa cells stained with TA300537 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a significantly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.


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