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Anti-FOXO3A PHOSPHO Antibody
Also for FOXO3A (NM_001455)
|The antiserum was produced against synthesized phosphopeptide derived from humanFKHRL1 around the phosphorylation site of serine 253 (A-V-SP-M-D).|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500~1:1000, IHC: 1:50~1:100
|phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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Western blot analysis of extracts from NIH/3T3 cells using FKHRL1 antibody (Lanes 1 and 2) and FKHRL1 (phospho-Ser253) antibody (Lanes 3 and 4).
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody.