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Anti-FKHR PHOSPHO Antibody
Also for FKHR (NM_002015)
|The antiserum was produced against synthesized phosphopeptide derived from humanFKHR around the phosphorylation site of serine 319 (T-S-SP-N-A).|
|Human, Mouse, Rat
||WB: 1:500~1:1000, IHC: 1:50~1:100
|phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. (Protein A or G Sepharose)
|Homo sapiens forkhead box O1 (FOXO1)|
|FKH1; FKHR; FOXO1A|
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
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Western blot analysis of extracts from 293 cells (10% serum-treated, 15min) and HeLa cells (EGF-treated, 200ng/ml, 15min), using FKHR antibody (Lanes 1, 2 and 3) and FKHR (phospho-Ser319) antibody (Lanes 4 and 5).
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FKHR (phospho-Ser319) antibody.