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Anti-FOS PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from human FOS around the phosphorylation site of threonine 232 (V-A-TP-P-E).|
|Human (Identities = 100%, Positives = 100%); Mouse (Identities = 84%, Positives = 92%);Rat (Identities = 84%, Positives = 84%)
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1: 20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|FOS (Phospho-Thr232) antibody detects endogenous levels of FOS only when phosphorylated at threonine 232.
|Homo sapiens FBJ murine osteosarcoma viral oncogene homolog (FOS)|
|AP-1; C-FOS; p55|
EGFR1 Signaling Pathway
MAPK signaling pathway
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
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Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 5mins), using FOS (Phospho-Thr232) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from COS7 cells (Lane 2), using FOS (Phospho-Thr232) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FOS (Phospho-Thr232) antibody.The picture on the right is treated with the synthesized peptide.