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Anti-FN1 Antibody F1
|A recombinant protein was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||IHC-Fr: 1:250; WB: 1:50000; IHC-P: Use at an assay dependent dilution; IP: 1:50; ICC/IF: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
|Homo sapiens fibronectin 1 (FN1), transcript variant 3|
|CIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF|
|This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq]. |
Senescence and Autophagy
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Western blot - Fibronectin antibody [F1]; Anti-Fibronectin antibody [F1] at 1/50000 dilution + human plasma.Predicted band size : 263 kDa.Additional bands at : 240 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - Fibronectin antibody [F1]; Immunohistochemical analysis of fibronectin in paraffin embedded human stomach tissue, using ab32419 at 1/250.
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1]; ICC/IF image of ab32419 stained human mesenchymal stem cells. The cells were fixed in paraformaldehyde and then incubated in 0.1%BSA / 1% goat serum for 30 minutes, to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 2 hours at 22°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG. DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1]; ICC/IF image of ab32419 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab32419 at 1/100 dilution overnight at +4°C. The secondary antibody (green)was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.