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Anti-FN1 Antibody F1
|A recombinant protein was used as immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||IHC-Fr: 1:250; WB: 1:50000; IHC-P: Use at an assay dependent dilution; IP: 1:50; ICC/IF: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
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Western blot - Fibronectin antibody [F1]; Anti-Fibronectin antibody [F1] at 1/50000 dilution + human plasma.Predicted band size : 263 kDa.Additional bands at : 240 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - Fibronectin antibody [F1]; Immunohistochemical analysis of fibronectin in paraffin embedded human stomach tissue, using TA303517 at 1/250.
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1]; ICC/IF image of TA303517 stained human mesenchymal stem cells. The cells were fixed in paraformaldehyde and then incubated in 0.1%BSA / 1% goat serum for 30 minutes, to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 2 hours at 22°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG. DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1]; ICC/IF image of TA303517 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody TA303517 at 1/100 dilution overnight at +4°C. The secondary antibody (green)was DyLight 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.