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Home Antibody All anti-FN1 antibodies

Anti-FN1 Antibody F1

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303517
  • Rabbit monoclonal antibody against Fibronectin(F1)
100ul 325 In Stock
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WB(1)
IHC(1)
IF(2)

OriGene Data

ImmunogenA recombinant protein was used as immunogen.
Clone NameF1 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsIHC-Fr: 1:250; WB: 1:50000; IHC-P: Use at an assay dependent dilution; IP: 1:50; ICC/IF: 1:100
Predicted MW Explanation 263 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens fibronectin 1 (FN1), transcript variant 3
Alternative NameCIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF
Database LinkNP_002017
Entrez Gene 2335 Human
FunctionThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq].
Related PathwaySecreted ProteinES Cell Differentiation/IPSDruggable Genome Focal adhesionECM-receptor interactionRegulation of actin cytoskeletonPathways in cancerSmall cell lung cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Fibronectin antibody [F1]; Anti-Fibronectin antibody [F1] at 1/50000 dilution + human plasma.Predicted band size : 263 kDa.Additional bands at : 240 kDa. We are unsure as to the identity of these extra bands.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Fibronectin antibody [F1]; Immunohistochemical analysis of fibronectin in paraffin embedded human stomach tissue, using TA303517 at 1/250.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1]; ICC/IF image of TA303517 stained human mesenchymal stem cells. The cells were fixed in paraformaldehyde and then incubated in 0.1%BSA / 1% goat serum for 30 minutes, to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 2 hours at 22°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG. DAPI was used to stain the cell nuclei (blue).
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1]; ICC/IF image of TA303517 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody TA303517 at 1/100 dilution overnight at +4°C. The secondary antibody (green)was DyLight 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

 

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