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Anti-FLT1 Antibody Y103
Also for FLT1 (NM_002019)
|A synthetic peptide corresponding to residues in N-terminus of human VEGFR1 was used as immunogen. The antibody can detect both VEGFR1 and its splice isoform sFlt1.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:5000; IHC-P: 1:250; ICC/IF: 1:250; IP: 1:100; FC: 1:1000; IHC-Fr: 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Homo sapiens fms-related tyrosine kinase 1 (FLT1), transcript variant 1|
|FLT; FLT-1; VEGFR-1; VEGFR1|
|Oncogene FLT belongs to the src gene family and is related to oncogene ROS (MIM 165020). Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The sequence structure of the FLT gene resembles that of the FMS gene (MIM 164770); hence, Yoshida et al. (1987) proposed the name FLT as an acronym for FMS-like tyrosine kinase.[supplied by OMIM]. |
Cytokine-cytokine receptor interaction
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Western blot - VEGF Receptor 1 antibody [Y103]; Anti-VEGF Receptor 1 antibody [Y103] at 1/10000 dilution + mouse brain tissue.Predicted band size : 151 kDa.Observed band size : 180 kDa .
Immunohistochemistry (Paraffin-embedded sections) - VEGF Receptor 1 antibody [Y103]; Immunohistochemical analysis of VEGF receptor 1 expression in paraffin-embedded skin cancer, using 1/250 ab32152.
Immunocytochemistry/ Immunofluorescence - VEGF Receptor 1 antibody [Y103]; Immunofluorescent analysis of VEGF receptor 1 expression in A431 cells using 1/250 ab32152.
Immunocytochemistry/ Immunofluorescence - VEGF Receptor 1 antibody [Y103]; ab32152 at 1/200 dilution staining VEGF Receptor 1 in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used as secondary at 1/500 dilution.
Flow Cytometry - Anti-VEGF Receptor 1 antibody [Y103]; Overlay histogram showing A431 cells stained with ab32152 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.