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Anti-FLNA PHOSPHO Antibody EP2309Y
Also for FLNA (NM_001456)
|A phospho specific peptide corresponding to residues surrounding serine 1458 of human Filamin A was used as an immunogen. This antibody detects Filamin A phosphorylated at serine 1458.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:5000; IHC-P: 1:50 - 1:100; ICC/IF: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens filamin A, alpha (FLNA), transcript variant 1|
|ABP-280; ABPX; CSBS; CVD1; FLN; FLN-A; FLN1; FMD; MNS; NHBP; OPD; OPD1; OPD2; XLVD; XMVD|
|Actin-binding protein, or filamin, is a 280-kD protein that crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes, and second messengers.[supplied by OMIM]. |
MAPK signaling pathway
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Western blot - Filamin A (phospho S1458) antibody [EP2309Y]; All lanes : Anti-Filamin A (phospho S1458) antibody [EP2309Y] at 1/5000 dilution.Lane 1 : HeLa cell lysate - untreated.Lane 2 : HeLa cell lysate - treated with AP.Lysates/proteins at 10 µg per lane.Secondary.HRP-labelled Goat anti-Rabbit antibody at 1/2000 dilution.Predicted band size : 280 kDa.Observed band size : 260 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Filamin A (phospho S1458) antibody [EP2309Y]; Immunohistochemical analysis of paraffin-embedded human stomach (left) and human uterus tissue (right) using 1/50 ab68424. Heat-mediated antigen retrieval was performed prior to commencing with IHC protocol and an HRP-conjugated polymerized secondary antibody was used.