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Anti-FLNA PHOSPHO Antibody EP2310AY
Also for FLNA (NM_001456)
|A phospho specific peptide corresponding to residues surrounding serine 2151 was used as an immunogen. This antibody detects filamin A phosphorylated on serine 2151.|
||Tissue culture supernatant
|Human (Does not react with: Mouse)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||ICC/IF: Use at an assay dependent concentration; ELISA: Use at an assay dependent concentration; WB: 1:10000 - 1:20000; ICC: 1:100 - 1:200; IHC-P: 1:100 - 1:200
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens filamin A, alpha (FLNA), transcript variant 1|
|ABP-280; ABPX; CSBS; CVD1; FLN; FLN-A; FLN1; FMD; MNS; NHBP; OPD; OPD1; OPD2; XLVD; XMVD|
Entrez Gene 2316 Human
|Actin-binding protein, or filamin, is a 280-kD protein that crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes, and second messengers.[supplied by OMIM]. |
| MAPK signaling pathwayFocal adhesion|
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Western blot - Filamin A (phospho S2151) antibody [EP2310AY]; All lanes : Anti-Filamin A (phospho S2152) antibody [EP2310AY] at 1/20000 dilution.Lane 1 : HeLa lysate, untreated.Lane 2 : HeLa lysate, treated with calyculin A.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 281 kDa.Observed band size : 281 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Filamin A (phospho S2151) antibody [EP2310AY]; TA303514 at 1/100 dilution staining Filamin A in human uterus by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Filamin A (phospho S2152) antibody [EP2310AY]; Immunofluorescence analysis of U. parvum infected BPH-1 (Human benign prostate) cells staining Filamin A (phospho S2152) with TA303514 (red). Cells were fixed with formaldehyde before incubating with primary antibody. An AlexaFluor?594-conjugated goat anti-rabbit IgG was used as the secondary antibody.