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Anti-FLNA PHOSPHO Antibody EP2310AY
Also for FLNA (NM_001456)
|A phospho specific peptide corresponding to residues surrounding serine 2151 was used as an immunogen. This antibody detects filamin A phosphorylated on serine 2151.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC/IF: Use at an assay dependent concentration; ELISA: Use at an assay dependent concentration; WB: 1:10000 - 1:20000; ICC: 1:100 - 1:200; IHC-P: 1:100 - 1:200
|Does not react with Mouse. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens filamin A, alpha (FLNA), transcript variant 1|
|ABP-280; ABPX; CSBS; CVD1; FLN; FLN-A; FLN1; FMD; MNS; NHBP; OPD; OPD1; OPD2; XLVD; XMVD|
|Actin-binding protein, or filamin, is a 280-kD protein that crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes, and second messengers.[supplied by OMIM]. |
MAPK signaling pathway
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Western blot - Filamin A (phospho S2151) antibody [EP2310AY]; All lanes : Anti-Filamin A (phospho S2152) antibody [EP2310AY] at 1/20000 dilution.Lane 1 : HeLa lysate, untreated.Lane 2 : HeLa lysate, treated with calyculin A.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 281 kDa.Observed band size : 281 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Filamin A (phospho S2151) antibody [EP2310AY]; ab75978 at 1/100 dilution staining Filamin A in human uterus by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Filamin A (phospho S2152) antibody [EP2310AY]; Immunofluorescence analysis of U. parvum infected BPH-1 (Human benign prostate) cells staining Filamin A (phospho S2152) with ab75978 (red). Cells were fixed with formaldehyde before incubating with primary antibody. An AlexaFluor?594-conjugated goat anti-rabbit IgG was used as the secondary antibody.