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Anti-FLNA Antibody EP2405Y
Also for FLNA (NM_001456)
|A synthetic peptide corresponding to residues near the C-terminus of human Filamin A was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: Use at an assay dependent concentration; WB: 1:250000 - 1:500000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens filamin A, alpha (FLNA), transcript variant 1|
|ABP-280; ABPX; CSBS; CVD1; FLN; FLN-A; FLN1; FMD; MNS; NHBP; OPD; OPD1; OPD2; XLVD; XMVD|
|Actin-binding protein, or filamin, is a 280-kD protein that crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes, and second messengers.[supplied by OMIM]. |
MAPK signaling pathway
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Western blot - Filamin A antibody [EP2405Y] - Carboxyterminal end; All lanes : Anti-Filamin A antibody [EP2405Y] - Carboxyterminal end at 1/500000 dilution.Lane 1 : COS-1 cell lysate.Lane 2 : Hela cell lysate.Lane 3 : 3T3 cell lysate.Lane 4 : C6 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 281 kDa.Observed band size : 281 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Filamin A antibody [EP2405Y] - Carboxyterminal end; Immunohistochemical analysis of paraffin-embedded human uterus using ab76289 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Filamin A antibody [EP2405Y] - Carboxyterminal end; Immunofluorescent staining of HeLa cells using ab76289 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Filamin A antibody [EP2405Y] - Carboxyterminal end; Immunofluorescence analysis of BPH-1 cells, staining Filamin A with ab76289.
Flow Cytometry - Anti-Filamin A antibody [EP2405Y] - Carboxyterminal end; Overlay histogram showing A431 cells stained with ab76289 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.